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A positive indirect ELISA result requires

A positive indirect ELISA result requires

  • one known antibody, one unknown antibody, and an unknown antigen.
  • a known antigen, an unknown antibody, and a known antibody
  • two known antibodies and one unknown antigen.
  • two known antibodies and one known antigen.
  • All of the choices are correct



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1 Answer

  1. A positive indirect ELISA result requires:

    • A known antigen
    • An unknown antibody (which is what you’re testing for in the sample)
    • A known antibody (secondary antibody that binds to the unknown antibody if it is present)

    Given the options, the correct answer is:

    b) a known antigen, an unknown antibody, and a known antibody

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